杭州仟诺生物科技有限公司17504-044B-27® 添加剂是无血清添加剂 N2等常用试剂大量现货备货,到货
B-27® 添加剂是无血清添加剂,用于海马神经元和其他中枢神经系统(CNS)神经元的生长和保持其短期或长期活性。B-27® 添加剂是50X 的液体,可以与Neurobasal® 培养基或Neurobasal®-A 培养基组合使用,用于神经细胞培养而不需要再添加饲养层细胞。
B-27® 添加剂适和应用于大多数神经细胞培养。对于特别的应用,我们提供 其他配方的B-27® 添加剂.
质量控制检测
B-27® 添加剂 进行了18天妊娠的原代大鼠(Sprague Dawley) 的胚胎海马神经元生长检测。 通过了细菌和真菌的检测. B-27® 也做了1X 浓度的内毒素水平测试。
仅供研究使用。除非另有说明,不得用于任何动物或人类治疗或诊断。
B-27® 添加剂是无血清添加剂
常用规格
Cell Type: | Neuronal Cells |
Product Size: | 10 ml |
Classification: | Serum-Free |
Other Additives: | Vitamin A, Antioxidant Cocktail, Insulin |
Purity or Quality Grade: | Cell Culture Grade |
Green Features: | Sustainable packaging |
Shipping Condition: | Dry Ice |
Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
引文和参考文献 (17)
D2 receptors inhibit the secretory process downstream from calcium influx in dopaminergic neurons: implication of K+ channels.
作者
Congar P; Bergevin A; Trudeau LE
杂志
J Neurophysiol (2002) 87:1046-1102
产品用途
Using a culture system in which isolated DAergic neurons establish self-innervating synapses ("autapses") that release both DA and glutamate, we studied the mechanism by which presynaptic D2 receptors inhibit glutamate-mediated excitatory postsynaptic currents (EPSCs).
ID:
PN64425
Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice.
作者
Di Paolo Gilbert; Sankaranarayanan Sethuraman; Wenk Markus R; Daniell Laurie; Perucco Ezio; Caldarone Barbara J; Flavell Richard; Picciotto Marina R; Ryan Timothy A; Cremona Ottavio; De Camilli Pietro;
杂志
Neuron (2002) 33:789-804
产品用途
Primary cultures of cortical neurons were prepared. Cells were maintained up to 3 weeks in Neurobasal/B27 medium at 37°C in a 5% CO2 humidified incubator.
ID:
PN47418
Blocking HES1 expression initiates GABAergic differentiation and induces the expression of p21(CIP1/WAF1) in human neural stem cells.
作者
Kabos Peter; Kabosova Andrea; Neuman Toomas;
杂志
J Biol Chem (2002) 277:8763-8769
产品用途
Resected human fetal brain tissue was placed into ice cold DMEM/F-12 with penicillin/streptomycin for further dissection. The tissue was cut into small pieces and trypsinized (0.02 mg/ml trypsin in versene (Invitrogen) at 37 °C for 10 min. Cell suspension was centrifuged at 400 rpm for 5 min, the pellet was washed once with DMEM/F-12 and plated at a density of 5000-10,000 viable cells/ml in the growth medium DMEM/F-12, B27 supplement (Invitrogen). Cells were washed with PBS (Invitrogen)
ID:
PN63471
Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death.
作者
Lesuisse Christian; Martin Lee J;
杂志
J Neurobiol (2002) 51:9-23
产品用途
A long-term cell culture system was used to study maturation, aging, and death of cortical neurons.
ID:
PN60461
An improved method for the expression of TRH in serum-supplemented primary cultures of fetal hypothalamic cells.
作者
Joseph-Bravo Patricia; Pérez-MartÃnez Leonor; Lezama Leticia; Morales-Chapa Claudia; Charli Jean Louis;
杂志
Brain Res Brain Res Protoc (2002) 9:93-104
产品用途
Primary cultures of dispersed cells from fetal nervous tissue are extensively used for studying multiple neuronal properties
ID:
PN62028